How do you use a trizol?
Add 0.2 ml of chloroform per 1 ml of TRIZOL Reagent. Cap sample tubes securely. Shake tubes vigorously by hand for 15 seconds and incubate them at 15 to 30°C for 2 to 3 minutes. Centrifuge the samples at no more than 12,000xg for 15 minutes at 2 to 8°C.
How do you quantify Microrna?
Currently, quantitative real-time PCR (qPCR), microarray, and next-generation sequencing (NGS) are the most commonly used techniques for quantifying miRNA. While such procedures are routinely used in the laboratory, not one is completely ideal for rapid, reproducible miRNA detection.
How do you use QIAzol?
Add QIAzol Lysis Reagent to each tube: 1 ml QIAzol Lysis Reagent per 100 mg tissue is required. The volume of tissue should not exceed 10% of the volume of QIAzol Lysis Reagent. ▲ Place the tubes in the TissueLyser Adapter Set 2 x 24. Operate the TissueLyser II for 2 min at 20 Hz.
Which rneasy mini kit should I use to extract RNA from tissue?
The RNeasy Fibrous Tissue Kit is optimized for use with fiber-rich animal tissues. For extraction of RNA from plant tissue we recommend the RNeasy Plant Mini Kit (cat. no. 74904). What is the smallest sample size that can be used with RNeasy Mini Kits?
What is the rneasy mini kit?
The RNeasy Mini Kit allows efficient purification of total RNA from small amounts of starting material. RNeasy technology simplifies total RNA isolation by combining the stringency of guanidine-isothiocyanate lysis with the speed and purity of silica-membrane purification.
What samples can I use the rneasy plus micro kit for?
The RNeasy Plus Micro Kit is suitable for small samples, including: 1 Laser-microdissected cryosections 2 Fine-needle aspirates More
How does the rneasy micro procedure work?
The RNeasy Micro procedure uses a novel technology to purify RNA from small amounts of tissues or cells (as little as 1 cell). Can the QuantiTect Whole Transcriptome Kit be used for amplification of RNA from LCM samples?