How does BODIPY tr ceramide work?
The BODIPY TR Ceramide in this kit is supplied encapsulated in SomaServe’s PolyNaut™ particles. PolyNaut particles are non-toxic, pH-sensitive vesicles which are used to deliver cargos into the cell without the need for solvents and with no effect on metabolic activity.
Is BODIPY cell permeable?
However, the big challenge is the poor cell membrane permeability of BODIPY dyes, which limits their application in cell biological imaging [16,19].
Is BODIPY polar?
Yet, due to the nonpolar nature of BODIPY core, they tend to aggregate in highly polar solvents . The intermolecular interactions are known to strongly affect the photophysical properties of different fluorophores by the undesired effect of fluorescence quenching due to the π-π stacking among others [9,10,11].
Is bodipy hydrophobic?
BODIPY FL dye is a green fluorescent dye with unique hydrophobic properties ideal for staining lipids, membranes, and other lipophilic compounds.
Is Tamra cell permeable?
Fluorescent probes 2 and 3, derivatives of the xanthene-based TAMRA fluorophore, showed even cellular distribution throughout the cytosol, and also permeate nuclear space to a large extent.
What does bodipy stand for?
The common name is an abbreviation for “boron-dipyrromethene”. It is a red crystalline solid, stable at ambient temperature, soluble in methanol. BODIPY. Names.
What does bodipy stain for?
With its nonpolar structure and long-wavelength absorption and fluorescence, BODIPY™ 493/503 can be used as a stain for neutral lipds and as a tracer for oil and other nonpolar lipids.
Are ceramides harmful?
Like cholesterol, ceramides are sticky, greasy molecules that help maintain cell membranes and perform other critical life-sustaining tasks. In excessive amounts, both substances can wreak havoc on the cardiovascular system, promoting the accumulation of plaque in arterial walls.
Do ceramides really work?
Ceramides help create a barrier to prevent permeability. This locks moisture into your skin, which helps prevent dryness and irritation. It may also help protect your epidermis from environmental damage. These benefits may have anti-aging effects.
How do you store bodipy?
- Prepare 5 mM BODIPY stock solution. Dissolve 1.3 mg BODIPY in 1 ml DMSO and can be stored at −20 °C.
- 2 μM BODIPY staining solution can be prepared by diluting stock solution 1:2,500 in PBS.
Is Cy5 cell permeable?
Cy5, BODIPY650/665, and SiR650 are highly cell permeable, and thus we anticipated that activated fluorescence probes based on them would become cell permeable after MMP enzymatic reaction.
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