What is the function of HGPRT gene?
The HPRT1 gene provides instructions for producing an enzyme called hypoxanthine phosphoribosyltransferase 1. This enzyme allows cells to recycle purines, a type of building block of DNA and its chemical cousin RNA.
What syndrome causes by a point mutation in codon 200 in the HPRT1 gene?
Lesch-Nyhan disease is an X-linked recessive disorder that is caused by a wide variety of mutations in the HPRT1 gene. The gene encodes hypoxanthine-guanine phosphoribosyl transferase, an enzyme involved in purine metabolism.
What is HGPRT in biochemistry?
Hypoxanthine-guanine phosphoribosyltransferase deficiency. Hypoxanthine-guanine phosphoribosyltransferase (HPRT) is the enzyme which catalyzes salvage of the purine bases guanine and hypoxanthine into their respective monophosphate nucleoside i.e., guanylic monophosphate (GMP) and inosine monophosphate (IMP).
What is the HGPRT status in a hybridoma cell?
Hybridomas are immortal (immune to cellular senescence), HGPRT+ cells that result from fusion of mortal, HGPRT+ plasma cells and immortal, HGPRT− myeloma cells. They are created to produce monoclonal antibodies in biotechnology.
Where is HGPRT expressed?
69.1). HPRT is ubiquitously expressed in cells of the human body, with highest levels found in the basal ganglia. The subcellular localization is cytoplasmic. IMP and GMP are produced from the de novo pathway, as well as from the HPRT-catalyzed salvage pathway.
What are good housekeeping genes?
What is a Good Housekeeping Gene? The definition of a good housekeeping gene, is one that has stable expression in varying conditions, thus can be used to normalize other influences which may skew the result.
How is Lesch Nyhan syndrome inherited?
Lesch-Nyhan syndrome is inherited in an X-linked pattern. X-linked genetic disorders are conditions caused by an abnormal gene on the X chromosome and manifest mostly in males. Females that have an abnormal gene present on one of their X chromosomes are carriers for that disorder.
Where is HGPRT?
This gene is located on the X chromosome and is hemizygous in CHO cells (hprt+/–). This enzyme is involved in the purine recycling pathway. It catalyzes the reaction of hypoxanthine or guanine with 5-phospho-a-D-ribosyl-1-pyrophosphate (PRPP) to produce either inosine 5′-monophosphate or guanosine 5′-monophosphate.
Do B-cells have HGPRT?
Unfused B cells also die as they have a short life span. Thus, only the B cell-myeloma hybrids survive because the HGPRT gene in B cells is functional. MAbs are widely used for the prevention, diagnosis, and treatment of diseases.
Where is HGPRT found?