AdministratorWhat is the principle of micellar electrokinetic capillary chromatography?
In micellar electrokinetic capillary chromatography, surfactants are added to the buffer solution in concentration above their critical micellar concentrations, consequently micelles are formed; micelles that undergo electrophoretic migration like any other charged particle.
What is the principle of GLC chromatography?
Principle. GLC is based upon partitioning of compounds between stationary liquid and mobile gas phase. Due to its high sensitivity, reproducibility, and speed of resolution, it is widely used for several qualitative and quantitative analyses.
What is the principle of liquid chromatography?
Chromatography is used to separate proteins, nucleic acids, or small molecules in complex mixtures. Liquid chromatography (LC) separates molecules in a liquid mobile phase using a solid stationary phase. Liquid chromatography can be used for analytical or preparative applications.
What are the four types of liquid chromatography?
Depending on the choice of stationary and mobile phase, four major separation modes are used to separate most compounds.
- Reversed-Phase Chromatography.
- Normal Phase Chromatography.
- Ion Exchange Chromatography.
- Size Exclusion Chromatography.
Why can micellar electrokinetic chromatography be used to separate neutral molecules?
In MEKC the separation of neutral solutes is due to the interaction of solutes with the hydrophobic core of ionic micelles, which are formed when surfactants are present at concentrations above their critical micelle concentration (CMC).
What is GLC and HPLC?
HPLC and GC are both methods of separation of compounds from a mixture. While HPLC refers to High Pressure Liquid Chromatography, GC is simply Gas Chromatography.
What type of chromatography is GLC?
Gas chromatography—also referred to as gas-liquid chromatography (GLC)—is a specific type of chromatography that utilizes an inert gaseous mobile phase and a liquid stationary phase.
What is liquid chromatography and its types?
Types of liquid chromatography include: Liquid-solid chromatography. Describing both normal and reverse phase chromatography, the stationary phase is a column made up of alumina or silica based compound that allows the liquid mobile phase to absorb or pass through it.
What is the other name for liquid chromatography?
High-performance liquid chromatography (HPLC), formerly referred to as high-pressure liquid chromatography, is a technique in analytical chemistry used to separate, identify, and quantify each component in a mixture.