What does Alexa Fluor 488?

What does Alexa Fluor 488?

Invitrogen Alexa Fluor 488 dye is a bright, green-fluorescent dye with excitation ideally suited to the 488 nm laser line….Alexa Fluor 488 protein/antibody labeling kits.

Target protein Kit Cat. No.
Site-specific antibody labeling
1 mg antibody/protein Alexa Fluor 488 Antibody/Protein Labeling Kit A10235

Is Alexa Fluor 488 same as FITC?

Molecular Probes™ Alexa Fluor® 488 dye—with nearly identical spectral properties and quantum yield as fluorescein (FITC)—produces brighter, more photostable conjugates that are ideal for imaging and other applications requiring increased sensitivity and environmentally insensitive fluorescence detection.

What color is Alexa Fluor 488?

green fluorophore
Alexa Fluor® 488 is a green fluorophore commonly used in applications such as immunolabeling, fluorescence microscopy, and flow cytometry. The dye has an absorption wavelength that peaks around 496 nm, and an emission maximum around 519 nm.

What is Alexa Fluor used for?

Alexa Fluor dyes are frequently used as cell and tissue labels in fluorescence microscopy and cell biology. Alexa Fluor dyes can be conjugated directly to primary antibodies or to secondary antibodies to amplify signal and sensitivity or other biomolecules.

How long do Alexa Fluor antibodies last?

2 weeks
​What temperature should I store the antibody at? Storage at 4°C should not exceed 1 or 2 weeks.

Can I use FITC and PE together?

FITC overlap a lot with Pe, don’t use them together on same cells. PerCP-‐Cy5. 5 overlap surprisingly a lot with BV711, don’t use together.

What does FITC stain for?

Fluorescein-5-isothiocyanate (FITC) is a fluorescence dye and belongs to the xanthene dyes. FITC is used for labeling of different biomolecules, e.g. immunoglobulins, lectins and other proteins, peptides, nucleic acids, nucleotides; oligo-and polysaccha- rides.

What is fluorescein sodium used for?

Fluorescein sodium, the sodium salt of fluorescein, is used extensively as a diagnostic tool in the field of ophthalmology and optometry, where topical fluorescein is used in the diagnosis of corneal abrasions, corneal ulcers and herpetic corneal infections.

Is FITC a 488?

High-performance alternatives to fluorescein The Invitrogen Molecular Probes Alexa Fluor 488 dye—with spectral properties and quantum yield nearly identical to those of fluorescein isothiocyanate (FITC)—produces brighter, more photostable conjugates.

What is Alexa Fluor® 488 azide?

The green-fluorescent Alexa Fluor® 488 azide is reactive with terminal alkynes via a copper-catalyzed click reaction. In addition to being an exceedingly bright and photostable fluorophore for use with flow cytometry, microscopy and HCS, Alexa Fluor® 488 can also be utilized as a bio-orthogonal or biologically unique hapten…

What is the emission maximum of Alexa Fluor 488 secondary antibodies?

The absorption at 495 nm and emission maximum at 519 nm spectrum make Alexa Fluor 488 secondary antibodies ideal to do fluorescent cell staining of multiple proteins with Alexa Fluor® 594 and Alexa Fluor® 647 conjugated secondary antibodies.

What is the Best labelling kit for Zenon Alexa Fluor 488?

Zenon Alexa Fluor 488 Mouse IgG2a Labeling Kit Z25102 <1–20 µg mouse IgG2b Zenon Alexa Fluor 488 Mouse IgG2b Labeling Kit Z25202 <1–20 µg human IgG Zenon Alexa Fluor 488 Human IgG Labeling Kit Z25402

What is Alexa Fluor dye?

Alexa Fluor dye family Alexa Fluor dyes are among the most trusted fluorescent dyes available today. Learn more about the: Brightness of Alexa Fluor dyes