What does microtubule acetylation do?
K40 acetylation protects microtubules from mechanical stress. . This acetylation increases the flexibility of the microtubule, which makes microtubules more resistant to mechanical stress, thus avoiding further breakage of the bent microtu- bule (2).
What is the common target of acetylation reaction?
The acetylation of proteins and peptides is believed to be mediated by an acetyl transferase, which catalyzes the transfer of an acetyl group from a donor molecule, acetyl-CoA, to the target protein or peptide.
What is the importance of acetylation?
Proteins that replicate DNA and repair damaged genetic material are created directly by acetylation. Acetylation also helps in DNA transcription. Acetylation determines the energy that proteins use during duplication and this determines the accuracy of copying the genes.
Why is tubulin a good target for anticancer drugs?
Tubulin and microtubules are the main targets of the Vinca alkaloids71, which depolymerize microtubules and destroy mitotic spindles at high concentrations (for example, 10–100 nM in HeLa cells71), therefore leaving the dividing cancer cells blocked in mitosis with condensed chromosomes.
How does acetylation of α-tubulin trigger apoptosis and autophage?
Reversible acetylation of α-tubulin has been implicated in modulating microtuble structures and functions, which may subsequently involve in cellular apoptosis and autophage. But how to trigger apoptosis or autophage at what level of acetylated α-tubulin (Ac-α-tubulin) are not known.
Does tubulin acetylation affect microtubule structure and stability?
Obviously, the biological role of tubulin acetylation does not require that this modification produce a direct effect on microtubule structure, stability or other intrinsic properties. Instead, it could operate to alter the binding of proteins that access the luminal surface of the microtubule.
Is acetylation of α-tubulin induced by tanespimycin involved in lung cancer?
Taken together, acetylation of α-tubulin induced by tanespimycin is involved in apoptosis and autophage in lung cancer cells.
What antibodies bind to acetylated tubulin?
This reagent is the only specific antibody that binds to acetylated tubulin. Other acetylation sites have been identified by enriching cell isolates using pan anti–acetylated lysine antibodies and mass spectrometry ( Choudhary et al ., 2009 ).