What is a cytokine array?

What is a cytokine array?

Cytokine arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte.

How does antibody microarray work?

An antibody microarray (also known as antibody array) is a specific form of protein microarray. In this technology, a collection of captured antibodies are spotted and fixed on a solid surface such as glass, plastic, membrane, or silicon chip, and the interaction between the antibody and its target antigen is detected.

What is proteome Profiler?

The Proteome Profiler Human Cytokine Array Kit is a membrane-based sandwich immunoassay. Samples are mixed with a cocktail of biotinylated detection antibodies (Step 1) and then incubated with the array membrane which is spotted in duplicate with capture antibodies to specific target proteins (Step 2).

What is human XL?

The Human XL Cytokine Array detects multiple cytokines, chemokines, growth factors and other soluble proteins in cell culture supernates. SW480 human colorectal adenocarcinoma cells were untreated or treated with 100 ng/mL of LPS for 24 hours. 500 µL of cell culture supernate was run on each array.

How do you test for cytokines?

Immunoassays: Immunoassays currently are the method of choice for determination of cytokines. Enzyme-linked immunosorbent assay (ELISA) is the commonly used form of immunoassay. ELISA uses a primary antibody for the capture and a secondary antibody conjugated to an enzyme or radioisotope for the detection.

How do you measure cytokines?

The enzyme-linked immunosorbant assay (ELISA) is the most popular way to detect cytokines and forms the basis for most other methods in use today. In its most basic form, a “capture” antibody on a solid support, generally one well of a 96-well plate, pulls cytokines out of a biological fluid such as serum.

What does protein microarray do?

A protein microarray (or protein chip) is a high-throughput method used to track the interactions and activities of proteins, and to determine their function, and determining function on a large scale. Its main advantage lies in the fact that large numbers of proteins can be tracked in parallel.

What is a protein microarray used for?

Analytical microarrays are typically used to profile a complex mixture of proteins in order to measure binding affinities, specificities, and protein expression levels of the proteins in the mixture. In this technique, a library of antibodies, aptamers, or affibodies is arrayed on a glass microscope slide.

What are normal cytokines?

Table 2

Cytokines (pg/ml) Group 1 (Age < 45) P-valuea
IL-6 2.91 ± 6.45 (0.16-37.7) 0.750
IL-8 23.9 ± 29.7 (4.2-132.6) 0.995
IP-10 462.2 ± 364.7 (145.3-2152.2) 0.673
MIP-1β 40.5 ± 38.8 (3.2-227.2) 0.633