AdministratorWhat is Cre transgenic mice?
A Cre mouse contains a Cre recombinase transgene under the direction of a tissue-specific promoter; a loxP mouse contains two loxP sites that flank a genomic segment of interest, the “floxed” locus. Typically, Cre and loxP mice are produced by using transgenic technology (Nagy 2000).
What is a Cre Deleter?
The Cre Deleter mouse was developed by Artemis Pharmaceuticals (now Taconic). The mutant was created by a targeted mutation knock in of Cre into the Gt(ROSA)26 locus. Cre is expressed under the control of the Gt(ROSA)26Sor gene.
What is CMV cre?
BALB/c-Tg(CMV-cre)1Cgn/J The RRID Number allows for easier identification of JAX and MMRRC strains in research publications. Common Name: CMV-Cre. These CMV-Cre mice express a transgene containing cre under the transcriptional control of a human cytomegalovirus minimal promoter.
What is genetic background in mice?
Genetic background: definition and examples As applied to a mutant mouse strain, genetic background refers to its genetic make-up (all its alleles at all loci) except the mutated gene of interest and a very small amount of other genetic material, generally from one or two other strains.
What is the purpose of Cre?
Cre recombinase plays important roles in the life cycle of the P1 bacteriophage. Upon infection of a cell the Cre-loxP system is used to cause circularization of the P1 DNA. In addition to this Cre is also used to resolve dimeric lysogenic P1 DNA that forms during the cell division of the phage.
How are Cre mice generated?
In this system, a region of interest flanked by two lox sites (floxed) is deleted or inverted by Cre-mediated recombination, leading to gene knockout only in a Cre-expressing cell. In general, Cre/lox mice are generated by mating a Cre-driver mouse with a flox mouse.
What is Cre knockout?
The Cre/lox system is one of the most powerful and versatile tools developed for mouse genetics. It gives mouse researchers sophisticated control over the location and timing of gene expression. Cre/lox is usually used to make knockout alleles, but it can also be used to activate gene expression.
How do you name a transgenic mouse?
Nomenclature for genetically engineered mice Alleles are designated either by the chemical mutagen or by the abbreviation “tm” (targeted mutation), followed by a number and the laboratory registration code, and selection criteria superscripted to the gene symbol (e.g., Apoa1tm1Unc).
How does Cre mice work?
What are Cre mice used for?
How to genotype Jax mice?
1. Evaluate the importance of genotyping when working with JAX mice 2. Determine why and when you need to genotype your JAX mice 3. Define different types of genotyping strategies: standard PCR, melt curve analysis, quantitative PCR and probe/endpoint analysis 4. Find and choose the correct genotyping protocol for your JAX mouse strain 5.
What is the genotype of my Mouse?
The genotype of your mouse determines the phenotype. Therefore, a homozygous mouse will have different characteristics than a heterozygous mouse. For this reason, it is important to routinely genotype your mice to maintain consistency with your mouse colonies.
What is the genotyping protocol for Gy mice?
Genotyping protocol for Gy mice (August, 2001) The Jackson Laboratory utilizes visible phenotype to genotype animals from the B6EiC3Sn-a/A-Gy (Stock Number 000503) colony. Animals carrying the allele display a circling behavior whereas animals do not.
Why is genotyping so important when working with mice?
An essential step in mouse breeding is genotyping mice to ensure that they have the expected genotype. Why is genotyping so important when working with mice in research, and when should you genotype your mouse? This course will help you successfully run a JAX protocol covering essential topics related to genotyping JAX mouse strains.