What is Ficoll-Paque?

What is Ficoll-Paque?

Ficoll-Paque Plus is a recognized standard in laboratories worldwide for the isolation of human lymphocytes for in vitro studies. Ficoll-Paque PLUS is a ready to use, sterile medium for isolation of lymphocytes in high yield from peripheral blood using a simple and rapid centrifugation procedure.

Does Ficoll-Paque expire?

All Answers (7) Doubts is true , use Ficoll-Paque should be within 12 weeks of opening , or before the expiration date as per the manufacturer’s instructions.

How do you dispose of Ficoll?

Disposal of this product, solutions and any by-products should at all times comply with the requirements of environmental protection and waste disposal legislation and any regional local authority requirements. Dispose of surplus and non-recyclable products via a licensed waste disposal contractor.

What does Ficoll 400 do?

Ficoll® 400 can be used for gradient centrifugation in all types of centrifuge rotors and for separation at unit gravity. For centrifugation, both discontinuous and continuous gradients are possible.

What is Ficoll Paque made of?

Ficoll-Paque PLUS is an aqueous solution of density 1.077 + 0.001 g/ml containing 5.7 g Ficoll 400 and 9 g sodium diatrizoate with 0.0231 g calcium disodium ethylenediamintetraacetic acid in every 100 ml.

Can Ficoll be frozen?

Conclusion: PBMCs isolated by leukapheresis and ficoll-hypaque treatment can be frozen with 10-percent DMSO in a -80 degrees C mechanical freezer.

What is Ficoll-Paque® reagents?

Manufactured under a Quality Management System certified to ISO 13485:2003. Ficoll-Paque® reagents are designed to prepare human mononuclear cells from peripheral blood, bone marrow, and umbilical cord blood via density gradient centrifugation.

What is the density of Ficoll-Paque plus?

Low levels of endotoxin (<0.12 EU/mL). Ficoll-Paque PLUS (density 1.077 g/mL) is a recognized standard in laboratories worldwide for the isolation of mononuclear cells for analytical research studies. There are no available product features to display for this product variant.

What is the Ficoll-Paque premium media used for?

Ficoll-Paque PREMIUM products are based on Ficoll-Paque PLUS, which has been used for more than 30 years as a sterile density medium for the isolation of high yields of mononuclear cells from bone marrow, peripheral blood, and cord blood.